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		McPackage Casual Observer
  
  Joined: 22 Jan 2007 Posts: 76 Location: California
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				 Posted: Thu Feb 08, 2007 12:23 pm    Post subject: Traveler J - "Catalyst (Find Him)" [02/08/2007] | 
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				Here's all the info discovered so far in the latest video, titled "Catalyst (Find Him)", which can be viewed at http://www.youtube.com/watch?v=egMFKctGzoE
 
 
The description is:
 
 
 	  | Quote: | 	 		  
 
If we value the pursuit of knowledge, we must be free to follow wherever that search may lead us.
 
 | 	  
 
 
The tags are:
 
 
 	  | Quote: | 	 		  | Search 5'3' Restriction FacilityJ OpAphid | 	  
 
 
The following were taken from the "parcel" thread:
 
 
 	  | McPackage wrote: | 	 		  In the new video, I believe the morse code is:
 
 
 	  | Code: | 	 		  
 
...-- ---.. ---.. ..-. ----. -
 
 | 	  
 
 
which translates to 388F9T
 
 | 	  
 
 
 	  | kellylen wrote: | 	 		  which is a tiny url
 
 
http://tinyurl.com/388F9T
 
 
the text at the tinyurl is a code
 
 
 	  | Code: | 	 		  R0dBR1RHQUdHR0dBR0NBR1RUR0dHQ0NBQUd
 
BVEdHQ0dHQ0NHQ0NHQUdHR0FDQ0dHVEdHR0NHQUN
 
HQ0dHR0FHVEdBR0dHR0FHQ0FHVFRHR0dDQ0FBR0FUR
 
0dDR0dDQ0dDQ0dBR0dHQUNDR0dUR0dHQ0dBQ0dHR0
 
dHQUdUR0FHR0FUQ0NUVFRUVEFUVENUVENHQUNUQ0F
 
HR0FUQ0NHR0dHQUdDQUdUVEdHR0NDQUFHQVRHR0N
 
HR0NDR0NDR0FHR0dBQ0NHR1RHR0dDR0FDR0dDR0dBR
 
1RHQUdHR0dBR0NBR1RUR0dHQ0NBQUdBVEdHQ0dHQ0
 
NHQ0NHQUdHR0FDQ0dHVEdHR0NHQUNHR0dHQUdUR0
 
FHR0dHQUdDQUdUVEdHR0NDQUFHQVRHR0NHR0NDR0N
 
DR0FHR0dBQ0NHR1RHR0dDR0FDR0NHR0dBR1RHDQoNC
 
jEoLTQpIDcoLTQpIDggMSAyIDMgOSgtMSkgNCgrMyk= | 	  
 
 | 	  
 
 
 
 	  | kellylen wrote: | 	 		  ok I did some more research.
 
 
base64 which converts to
 
 
 	  | Code: | 	 		  
 
GGAGTGAGGGGAGCAGTTGGGCCAAGAT
 
GGCGGCCGCCGAGGGACCGGTGGGCGACGCGG
 
GAGTGAGGGGAGCAGTTGGGCCAAGATGGCGGC
 
CGCCGAGGGACCGGTGGGCGACGGGGGAGTGAG
 
GATCCTTTTTATTCTTCGACTCAGGATCCGGGGAG
 
CAGTTGGGCCAAGATGGCGGCCGCCGAGGGACC
 
GGTGGGCGACGGCGGAGTGAGGGGAGCAGTTGG
 
GCCAAGATGGCGGCCGCCGAGGGACCGGTGGGC
 
GACGGGGAGTGAGGGGAGCAGTTGGGCCAAGATG
 
GCGGCCGCCGAGGGACCGGTGGGCGACGCGGGAGTG
 
 
1(-4) 7(-4) 8 1 2 3 9(-1) 4(+3)
 
 | 	  
 
 
which to me is a DNA sequence code. I'm not too sure about the numbers though | 	 
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		blahblablee Lonely Fan
 
  Joined: 31 Dec 2006 Posts: 237 Location: FacilityJ
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		blahblablee Lonely Fan
 
  Joined: 31 Dec 2006 Posts: 237 Location: FacilityJ
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				 Posted: Thu Feb 08, 2007 3:04 pm    Post subject:  | 
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				| Actually nvm it some of the things aren't in that code... | 
			 
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		blahblablee Lonely Fan
 
  Joined: 31 Dec 2006 Posts: 237 Location: FacilityJ
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		blahblablee Lonely Fan
 
  Joined: 31 Dec 2006 Posts: 237 Location: FacilityJ
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				 Posted: Thu Feb 08, 2007 4:14 pm    Post subject:  | 
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				Um the tag says to search restriction 5'3'
 
 
Restriction enzymes...These break up DNA
 
 
This leads me to believe that the numbers represent breaks in the DNA... | 
			 
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		McPackage Casual Observer
  
  Joined: 22 Jan 2007 Posts: 76 Location: California
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				 Posted: Fri Feb 09, 2007 3:59 pm    Post subject:  | 
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				One of the pages that comes up when searching for "5'3' restriction" is http://www.escience.ws/b572/L5/L5.htm .  I can't figure out if that is supposed to help or not, but it does look to be related to the "Coded Rings" video (see http://www.youtube.com/watch?v=0mM6Eo0VyG8 ).  Note that 2 of the tags on that video are "Decoder Ring".
 
 
Searching just for "5'3'" results in http://psyche.uthct.edu/shaun/SBlack/geneticd.html , which has a table very similar to the one used to decode the sequence in the previous puzzle.  I did go ahead and decode the sequence, but I still am not clear on what the numbers are for.  Here's the list, just in case it is relevant:
 
 
 	  | Code: | 	 		  
 
GGA   Glycine
 
GTG   Valine
 
AGG   Arginine
 
GGA   Glycine
 
GCA   Alanine
 
GTT   Valine
 
GGG   Glycine
 
CCA   Proline
 
AGA   Arginine
 
TGG   Tryptophan
 
CGG   Arginine
 
CCG   Proline
 
CCG   Proline
 
AGG   Arginine
 
GAC   Asparagine
 
CGG   Arginine
 
TGG   Tryptophan
 
GCG   Alanine
 
ACG   Threonine
 
CGG   Arginine
 
GAG   Aspartic acid
 
TGA   Ter [end]
 
GGG   Glycine
 
GAG   Aspartic acid
 
CAG   Glutamine
 
TTG   Leucine
 
GGC   Glycine
 
CAA   Glutamine
 
GAT   Aspartic acid
 
GGC   Glycine
 
GGC   Glycine
 
CGC   Arginine
 
CGA   Arginine
 
GGG   Glycine
 
ACC   Threonine
 
GGT   Glycine
 
GGG   Glycine
 
CGA   Arginine
 
CGG   Arginine
 
GGG   Glycine
 
AGT   Serine
 
GAG   Aspartic acid
 
GAT   Aspartic acid
 
CCT   Proline
 
TTT   Phenylalanine
 
TAT   Tyrosine
 
TCT   Serine
 
TCG   Serine
 
ACT   Threonine
 
CAG   Glutamine
 
GAT   Aspartic acid
 
CCG   Proline
 
GGG   Glycine
 
AGC   Serine
 
AGT   Serine
 
TGG   Tryptophan
 
GCC   Alanine
 
AAG   Lysine
 
ATG   Methionine
 
GCG   Alanine
 
GCC   Alanine
 
GCC   Alanine
 
GAG   Aspartic acid
 
GGA   Glycine
 
CCG   Proline
 
GTG   Valine
 
GGC   Glycine
 
GAC   Asparagine
 
GGC   Glycine
 
GGA   Glycine
 
GTG   Valine
 
AGG   Arginine
 
GGA   Glycine
 
GCA   Alanine
 
GTT   Valine
 
GGG   Glycine
 
CCA   Proline
 
AGA   Arginine
 
TGG   Tryptophan
 
CGG   Arginine
 
CCG   Proline
 
CCG   Proline
 
AGG   Arginine
 
GAC   Asparagine
 
CGG   Arginine
 
TGG   Tryptophan
 
GCG   Alanine
 
ACG   Threonine
 
GGG   Glycine
 
AGT   Serine
 
GAG   Aspartic acid
 
GGG   Glycine
 
AGC   Serine
 
AGT   Serine
 
TGG   Tryptophan
 
GCC   Alanine
 
AAG   Lysine
 
ATG   Methionine
 
GCG   Alanine
 
GCC   Alanine
 
GCC   Alanine
 
GAG   Aspartic acid
 
GGA   Glycine
 
CCG   Proline
 
GTG   Valine
 
GGC   Glycine
 
GAC   Asparagine
 
GCG   Alanine
 
GGA   Glycine
 
GTG   Valine
 
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		janesalteredstates Devoted Fan
  
  Joined: 12 Jan 2007 Posts: 763 Location: Jenlight's head
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				 Posted: Fri Feb 09, 2007 5:11 pm    Post subject:  | 
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				1(-4) 7(-4) 8 1 2 3 9(-1) 4(+3)
 
 
OK I am sure everyone tried this, but I have to post it.
 
 
1(-4) = -4
 
7(-4) = -28
 
then...
 
8 
 
1 
 
2 
 
3 
 
9(-1) = -9
 
4(+3) = 12 or +12 
 
 
-4 -28 8 1 2 3 -9 12
 
 
Can't hurt, right? _________________ “It takes a thousand voices to tell a single story. ”
 
http://youtube.com/profile?user=jenlight | 
			 
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		Brucker Casual Observer
  
  Joined: 13 Oct 2006 Posts: 91
 
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				 Posted: Fri Feb 09, 2007 6:25 pm    Post subject:  | 
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				 	  | McPackage wrote: | 	 		  |  I did go ahead and decode the sequence, but I still am not clear on what the numbers are for.  Here's the list, just in case it is relevant: | 	  
 
 
The problem with decoding DNA is that you have to know your starting point, which is why finding the 5'3' restriction is important. You've started decoding at the beginning, but even in real-life biology, DNA codes have strings of nonsense at the end. 
 
 
The link you gave names "GGATCC" as a 5'3' restriction (I don't know if it's the only one; as much as I do know about DNA, I'm not a molecular biologist, and didn't follow the article very well) and that sequence appears twice in the given sequence. If that were the sequence to look for, then you would start coding on the second guanosine, yielding:
 
 
 	  | Code: | 	 		  
 
GAT Aspartic acid
 
CCT Proline
 
TTT Phenylalanine
 
TAT Tyrosine
 
TCT Serine
 
TCG Serine
 
ACT Threonine
 
CAG Glutamine
 
GAT Aspartic acid
 
CCG Proline
 
GGG Glycine
 
AGC Serine
 
AGT Serine
 
TGG Tryptophan
 
GCC Alanine
 
AAG Lysine
 
ATG Methionine or START
 
GCG Alanine
 
GCC Alanine
 
GCC Alanine
 
GAG Glutamic acid
 
GGA Glycine
 
CCG Proline
 
GTG Valine
 
GGC Glycine
 
GAC Aspartic acid
 
GGC Glycine
 
GGA Glycine
 
GTG Valine
 
AGG Arginine
 
GGA Glycine
 
GCA Alanine
 
GTT Valine
 
GGG Glycine
 
CCA Proline
 
AGA Arginine
 
TGG Tryptophan
 
CGG Arginine
 
CCG Proline
 
CCG Proline
 
AGG Arginine
 
GAC Aspartic acid
 
CGG Arginine
 
TGG Tryptophan
 
GCG Alanine
 
ACG Threonine
 
GGG Glycine
 
AGT Serine
 
GAG Glutamic acid
 
GGG Glycine
 
AGC Serine
 
AGT Serine
 
TGG Tryptophan
 
GCC Alanine
 
AAG Lysine
 
ATG Methionine or START
 
GCG Alanine
 
GCC Alanine
 
GCC Alanine
 
GAG Glutamic acid
 
GGA Glycine
 
CCG Proline
 
GTG Valine
 
GGC Glycine
 
GAC Aspartic acid
 
GCG Alanine
 
GGA Glycine
 
GTG Valine
 
 | 	  
 
This looks like what you have actually, but starting later. Actually, the presence of those "START" codons could be important as well, but I can't make heads or tails of the numbers. _________________ -BRUCKER
 
 
Teen1: He's cool.
 
Teen2: Are you being sarcastic, dude?
 
Teen1: I don't even know anymore.
 
 
(The Simpsons, "Homerpalooza") | 
			 
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		Luminous Thor's Hammer
  
  Joined: 26 Nov 2006 Posts: 1359 Location: Facility J
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				 Posted: Sat Feb 10, 2007 6:27 pm    Post subject:  | 
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				I just wrote Traveler to ask why McPackage had to destroy J2, and he didn't do it himself. Here is the answer I received:
 
 
 
 
 
So it looks like "WD" are the initials of the man we are looking for.  It also looks like we must identify the right enzyme. That's pretty much out of my league - hope you biochemists can handle that one. He points to clues in the video - seems there must be some we have missed. What I find most curious is the last sentence:
 
 
"finding the enzyme is a double-edged sword if you don't realize that it can cut twice."
 
 
*runs off to rewatch the last video and see what I've missed* _________________ You made a wise choice, Bree.
 
There's no place like home.
 
Click to watch: The Ice Princess | 
			 
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		kellylen The Order of Denderah
  
  Joined: 21 Nov 2006 Posts: 2823 Location: New Jersey
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				 Posted: Sun Feb 11, 2007 12:43 am    Post subject:  | 
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				oiiiiiiii enzymes.
 
 
we would have to find the sequence for the proteins and then figure out which corresponds to the enzyme
 
 
as i just woke up im not going to do it.
 
 
maybe later today. _________________ -Kelly | 
			 
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		Luminous Thor's Hammer
  
  Joined: 26 Nov 2006 Posts: 1359 Location: Facility J
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				 Posted: Sun Feb 11, 2007 12:53 am    Post subject:  | 
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				Are there special enzymes that can "cut twice"? _________________ You made a wise choice, Bree.
 
There's no place like home.
 
Click to watch: The Ice Princess | 
			 
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		kellylen The Order of Denderah
  
  Joined: 21 Nov 2006 Posts: 2823 Location: New Jersey
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				 Posted: Sun Feb 11, 2007 2:05 am    Post subject:  | 
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				there maybe, I'm not quite sure yet as I only have just started studying biochemistry _________________ -Kelly | 
			 
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		blahblablee Lonely Fan
 
  Joined: 31 Dec 2006 Posts: 237 Location: FacilityJ
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				 Posted: Sun Feb 11, 2007 11:16 am    Post subject:  | 
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				 	  | Luminous wrote: | 	 		  | Are there special enzymes that can "cut twice"? | 	  
 
 
He's refering to the restriction enzymes:
 
 
From wikipedia:
 
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded DNA. The enzyme makes two incisions, one through each of the phosphate backbones of the double helix without damaging the bases. | 
			 
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		blahblablee Lonely Fan
 
  Joined: 31 Dec 2006 Posts: 237 Location: FacilityJ
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		TOSG Devoted Fan
  
  Joined: 14 Sep 2006 Posts: 651
 
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				 Posted: Sun Feb 11, 2007 2:25 pm    Post subject:  | 
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				Hey, I just stumbled upon this thread - I don't really know any of the backstory here, but I did a homology search on the DNA sequence, and it's a pretty good match with human nucleoporin (the protein complexes that allow molecules to enter and leave the cell's nucleus) DNA.
 
 
Don't know if that helps any, but there you go.
 
 
I do research in a molecular biology lab, so I'm pretty conversant with this stuff...feel free to PM me if you need.
 
 
EDIT: you guys also might want to try translating the DNA sequence into its one-letter protein codes, and see if it spells out anything.  I couldn't find anything, but maybe there's a trick (Anagram?  Special portion to decode?) | 
			 
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