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0201 -- "Opening the Box" [05/29/07]
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Shaneener
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PostPosted: Tue May 29, 2007 10:37 pm    Post subject: Reply with quote

Weren't there ceremony girls before Bree?

Why do they need all these girls?
Is one enough?

If the religion has been around for a while (which I'm assuming it has been), there had been other girls. With the same traits.

If the ceremony has to do with cloning/blood/ribozymes/etc. wouldn't they be satisfied with one girl? Why so many?

Thoughts?
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LittleChickie
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PostPosted: Tue May 29, 2007 10:40 pm    Post subject: Reply with quote

Maybe they haven't been able to get it right until now? Or they are hoping they can get it right this time. Science might not have been as far as it need to be before for the other ceremony girls to work, or completely work.
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Samara
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PostPosted: Tue May 29, 2007 10:42 pm    Post subject: Reply with quote

Quote:
Weren't there ceremony girls before Bree?


Going way back....way...way back....video #28:

Quote:

I've been chosen to participate in a ceremony. The ceremony is a really big deal in my religion. They only happen once in a really long while, and it's very, very difficult to attend. My parents won't even be allowed to come. It's a big honor, and it's going to take a lot of preparation. There's a bunch of stuff that I'm gonna have to memorize...and I'm really bad at memorizing, so...that should be fun. There's also gonna be some special exercises that I have to learn, and I don't really know how they work but my mom said she would help me with them. I'll have to go on a diet.
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RockinDuckie558
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PostPosted: Tue May 29, 2007 10:42 pm    Post subject: Reply with quote

Shaneener wrote:
Weren't there ceremony girls before Bree?

Why do they need all these girls?
Is one enough?

If the religion has been around for a while (which I'm assuming it has been), there had been other girls. With the same traits.

If the ceremony has to do with cloning/blood/ribozymes/etc. wouldn't they be satisfied with one girl? Why so many?

Thoughts?

Maybe the HoO is trying to figure out how to do all of those things. So they need a new girl for each generation. I'm just throwing ideas out there. I have no idea how this working out. I'm going to do some research and go to bed. Best of luck to you guys!
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Dsrtnomad
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PostPosted: Tue May 29, 2007 10:50 pm    Post subject: Reply with quote

I'm sorry I'm a total newbie at the forum, however here is a link to essentially the same image as in Bree's Dad's notebook. (sorry for not being able to quote the image from the earlier post, hopefully someone else can do that for me?)
http://www.aist.go.jp/aist_e/latest_research/2002/20020808/20020808.html
(despite the .jp ending, it is in english, not japanese, and how thrilled are they to get 500,000 hits tomorrow? Very Happy ).

How did I find this: google ribozyme reaction. How did I know to look for this: just got a C in intro to biochemistry and recognized the ribose structure (n+1 is just saying whatever purine/pyrimidine base it is, just means the structure that determines the specific code--which means the drawing is not anything specific, just a general idea of what ribozymes do) and saw the double-arrows which indicates a REVERSIBLE reaction. (Which means, that under certain circumstances it goes from right to left, under other conditions it goes left to right).

So dad's notes on this were just about ribozymes and how they function. I think we should look closer at the collage and graphs (when we get them from taylor --nudge nudge).
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Stellahella
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PostPosted: Tue May 29, 2007 10:54 pm    Post subject: Reply with quote

Gidget wrote:




whatdya think? Can anybody see anything else?


I think the word before "will?" is Epogen. And underneath "will" is --EFFECTS?

Also, I think it reads IRON SUPPLEMENTS, instead of it being supplement B or 13.

But I'm using my powers of squintation, so this could be wrong.
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mindinflight
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PostPosted: Tue May 29, 2007 10:58 pm    Post subject: Reply with quote

Hey y'all, if you head over to LG15 Today meepers_anonymous has done some amazing research on what was in the notebook!

Here's the image she found:


Looks a hell of a lot like that screenshot we have! It's called Hairpin Ribozyme Transesterification.

Just thought I'd put that out there.
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Samara
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PostPosted: Tue May 29, 2007 11:08 pm    Post subject: Reply with quote

Okay, so the sketch seems to have been identified at the hairpin roboszyme:





I've never heard of this...obviously...

but wikipedia has:

Quote:

The hairpin ribozyme, like the hammerhead ribozyme, is found in RNA satellites of plant viruses. It was first identified in the minus strand of the tobacco ringspot virus (TRSV) satellite RNA where it catalyses a self-cleavage reaction to process the products of rolling circle virus replication to unit-length satellite RNA. The structure consists of 2 domains, A and B. The 3' arm of domain A, which closes helices 1 and 2, contains the cleavage site and is linked to helix 3 of domain B by a linker of variable length and sequence.

[edit] Species distribution

The hairpin ribozyme has been identified in only 3 plant satellite RNAs:

* Tobacco ringspot virus (TRSV)
* chicory yellow mottle virus (CYMV)
* arabis mosaic virus (ARMV)



Plant viruses??
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SARAHPHOBIA88
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PostPosted: Tue May 29, 2007 11:08 pm    Post subject: Reply with quote

Meepers_Anonymous is so smart! Very Happy
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Dsrtnomad
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PostPosted: Tue May 29, 2007 11:10 pm    Post subject: Reply with quote

Gidget wrote:





I was able to pause it at a slightly better angle.

The bottom text starts out with

"DAY 2:
RIBOZYMES
RED BLOOD CELL COUNT
IRON SUPPLEMENTS"

the stuff in the top section is still fuzzy.

below the fingers it looks like:
...."DESIGN STUDY OF EPOGEN: -- WHY?
-- EFFECTS?
-- TRAIT REVERSAL?
-- " and the last one looks illegible.
it could be "SERINE?" or it could be "SERUM?"

at the top of the page, the last of the top line almost looks like WIZARDS OF VISION but that would make no sense. The last word could be VITAMIN as well, which would make sense (but not wizards.. it's after midnight and I need to get up at 6am tomorrow).

EDIT: because I figured out how to quote pictures. Smile
EDIT: because I figured out how to host my picture Smile

[URL=http://www.imagehosting.com] [/


Last edited by Dsrtnomad on Tue May 29, 2007 11:28 pm; edited 2 times in total
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Dsrtnomad
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PostPosted: Tue May 29, 2007 11:20 pm    Post subject: Reply with quote

Samara wrote:
Okay, so the sketch seems to have been identified at the hairpin roboszyme:



Aha, figured out the quoting thing, we'll see how it works.

Basically what this is doing is slicing up the RNA, and blocking it from reattaching (I'm assuming the ribozyme would also have a way to re-attach.)

The PO4 (+ - shaped thing in the middle) is what connects one nucleotide to the next (the parts that form the frame of the single helical chain of RNA). By moving the PO4 off of one ribose and attaching it to the only other possible spot for another phosphate (PO4) could attach, it ends the chain.
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WriterGirl
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PostPosted: Tue May 29, 2007 11:24 pm    Post subject: Reply with quote

I am horrible at science, so all of this stuff is practically Greek to me, but I have to say- this a much more interesting context then some textbook. Very Happy

The cloning theory (whatever version you want to accept) is looking more and more likely.

And I'm thinking there is most definitely some sort of code in the letter Bree's dad left her, but we probably need the entire thing to find it.

Am I the only one who's heart broke a little at the sight of the collage she made him? Or am I just a big ole softy? Crying or Very sad

I need to go to bed. I know I'll sign on in the morning to find, like, 200 pages, but so be it. Wink
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Samara
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PostPosted: Tue May 29, 2007 11:25 pm    Post subject: Reply with quote

Quote:
The PO4 (+ - shaped thing in the middle) is what connects one nucleotide to the next (the parts that form the frame of the single helical chain of RNA). By moving the PO4 off of one ribose and attaching it to the only other possible spot for another phosphate (PO4) could attach, it ends the chain.



I'm sorry, could you repeat that? There was this loud whooshing noise that distracted me.
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Dsrtnomad
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PostPosted: Tue May 29, 2007 11:35 pm    Post subject: Reply with quote

Samara wrote:
Quote:
The PO4 (+ - shaped thing in the middle) ==edited out biochemistry babble==

I'm sorry, could you repeat that? There was this loud whooshing noise that distracted me.


Yeah, that's why I got a C in the class. I learned it enough to get the gist, not able to really re-teach it to people. Embarassed

So think of the + with the P in the middle and the O's around it as tape. It's holding the vertical portions of the ladder together (the rungs of the ladder are the N+1 bits and are not important for what we're discussing). So the ribozyme is doing what Daniel described: cutting apart the RNA into sections, and the 'tape' is getting stuck to only one end of it, but you can't reuse that piece of tape again very easily. Hopefully that makes it easier to understand.

this is it for me until tomorrow morning


Last edited by Dsrtnomad on Tue May 29, 2007 11:40 pm; edited 1 time in total
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jodec123
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PostPosted: Tue May 29, 2007 11:38 pm    Post subject: Reply with quote

Quote:
Samara wrote:

I'm sorry, could you repeat that? There was this loud whooshing noise that distracted me.


LOL, I second that...I'm afraid I'm a bit over my head when it come to biology.


Last edited by jodec123 on Tue May 29, 2007 11:39 pm; edited 1 time in total
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